Flow injection spectrofluorimetric determination of cystine and cysteine
نویسندگان
چکیده
منابع مشابه
Flow Injection Spectrofluorimetric Determination of Cystine and Cysteine
Um procedimento relativamente simples e sensível com detecção espectrofluorimétrica foi desenvolvido para a determinação de cistina e cisteína por sistema de injeção em fluxo com determinação seqüencial. Esse método é fundamentado na redução de Tl(III) com cisteína em meio ácido, produzindo o reagente fluorescente TlCl 3 2(λ ex = 227 nm, λ em = 419 nm). Antes da injeção, a solução da amostra fo...
متن کاملThe chromatographic determination of cystine and cysteine residues in proteins as s-beta-(4-pyridylethyl)cysteine.
Disullide bonds in proteins and in seed meals were reduced with /3-mercaptoethanol to sulfhydryl groups. The generated sulfhydryl groups were then alkylated by treatment with 4-vinylpyridine. The cysteine residues were thus derivatized to S-(4-pyridylethyl)-L-cysteine residues. The cysteine derivative is stable to acid hydrolysis and on amino acid analysis by ion exchange elutes just before arg...
متن کاملFlow-injection determination of ascorbic acid and cysteine simultaneously with spectrofluorometric detection.
A simple and sensitive spectrofluorometric method was developed for the simultaneous determination of ascorbic acid and cysteine by a flow-injection system. This method is based on the reduction of Tl(III) with ascorbic acid or cysteine in acidic media, producing fluorescence reagent, TlCl3(2-) (lambdaex = 227 nm, lambdaem = 419 nm). The injected sample solution was divided into two separate st...
متن کاملElectrochemiluminescent determination of L-cysteine with a flow-injection analysis system.
A flow-injection electrochemiluminescent method for L-cysteine determination has been developed based on its enhancement of the electrochemiluminecence of luminol at a glassy carbon electrode. This method is simple and sensitive for cysteine determination. Under the selected experimental parameters, the linear range for cysteine concentration was 1.0 x 10(-6) - 5.0 x 10(-5) mol/l, and the detec...
متن کاملFlow injection spectrophotometric determination of N-acetyl-L-cysteine as a complex with palladium(II).
We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd(2+) ions in the concentration range from 1.0 × 10(-5) mol L(-1) to 6.0 × 10(-5) mol L(-1). The detection limit NAC was 5.84 × 10(-6) mol L(-1) and the recorded r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Brazilian Chemical Society
سال: 2009
ISSN: 0103-5053
DOI: 10.1590/s0103-50532009000200013